CJC-1295 · research summary

Activation of the GH/IGF-1 axis by CJC-1295, a long-acting GHRH analog, results in serum protein profile changes in normal adult subjects.

Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society · 2009. Summary drawn from the study's full text. Each statement is followed by the exact sentence it comes from. This is a research summary, not medical advice.

Study type

Proteomic analysis of serum samples before and after CJC-1295 administration
“Sera from 11 healthy young adult men before and one week after CJC-1295 injection were analyzed by two-dimensional gel electrophoresis for proteomic changes.”

Subjects

11 healthy young adult men with BMI lower than 25 kg/m2 and ages 20-34
“A subset of 11 healthy men that were part of a larger cohort previously described [8] were selected for this study. Individuals in this subset had a BMI lower than 25 kg/m2 (22.1 ± 1.6, mean ± SD), and ages 20-34 (25.2 ± 5.1, mean ± SD).”

Sample size

11 subjects
“A subset of 11 healthy men that were part of a larger cohort previously described [8] were selected for this study.”

Duration

One week between pre-treatment and post-treatment sampling
“Sera from 11 healthy young adult men before and one week after CJC-1295 injection were analyzed by two-dimensional gel electrophoresis for proteomic changes.”

Reported findings

Two protein spots decreased in intensity after treatment, identified as an apolipoprotein A1 isoform and a transthyretin isoform
“Two protein spots that displayed decreased intensities after treatment were identified as an apolipoprotein A1 isoform and a transthyretin isoform.”
Three protein spots increased in intensity, including beta-hemoglobin, a C-terminal fragment of albumin, and a mix of an immunoglobulin fragment and another C-terminal albumin fragment
“Three protein spots upregulated by CJC-1295 treatment included beta-hemoglobin, a C-terminal fragment of albumin, and a mix of an immunoglobulin fragment and another C-terminal albumin fragment.”
A linear relationship was found between the spot containing immunoglobulin and albumin fragments and IGF-1 levels
“A linear relationship was found between the spot containing immunoglobulin and albumin fragments and IGF-1 levels.”

Limitations stated by the authors

Molecular mechanisms linking identified proteins to GH and IGF-1 activity remain unclear, and it is not clear which specific protein in a mixed spot correlates with IGF-1
“Although the molecular mechanisms linking the identified proteins to GH and IGF-1 biological activity remain to be clarified, the results suggest that they represent potential biomarkers of GH and/or IGF-1 action.”

Source: PubMed 19386527 · DOI 10.1016/j.ghir.2009.03.001 · all study summaries

Subjects

11 healthy young adult men with BMI lower than 25 kg/m2 and ages 20-34

“A subset of 11 healthy men that were part of a larger cohort previously described [8] were selected for this study. Individuals in this subset had a BMI lower than 25 kg/m2 (22.1 ± 1.6, mean ± SD), and ages 20-34 (25.2 ± 5.1, mean ± SD).”

Reported findings

Two protein spots decreased in intensity after treatment, identified as an apolipoprotein A1 isoform and a transthyretin isoform

“Two protein spots that displayed decreased intensities after treatment were identified as an apolipoprotein A1 isoform and a transthyretin isoform.”

Three protein spots increased in intensity, including beta-hemoglobin, a C-terminal fragment of albumin, and a mix of an immunoglobulin fragment and another C-terminal albumin fragment

“Three protein spots upregulated by CJC-1295 treatment included beta-hemoglobin, a C-terminal fragment of albumin, and a mix of an immunoglobulin fragment and another C-terminal albumin fragment.”

A linear relationship was found between the spot containing immunoglobulin and albumin fragments and IGF-1 levels

“A linear relationship was found between the spot containing immunoglobulin and albumin fragments and IGF-1 levels.”

Limitations stated by the authors

Molecular mechanisms linking identified proteins to GH and IGF-1 activity remain unclear, and it is not clear which specific protein in a mixed spot correlates with IGF-1

“Although the molecular mechanisms linking the identified proteins to GH and IGF-1 biological activity remain to be clarified, the results suggest that they represent potential biomarkers of GH and/or IGF-1 action.”