GABA, Selank, and Olanzapine Affect the Expression of Genes Involved in GABAergic Neurotransmission in IMR-32 Cells.

In vitro gene expression analysis using qPCR in human neuroblastoma IMR-32 cells

“To test this hypothesis, we studied the changes in expression of 84 genes involved in the functioning of the GABAergic system and in the processes of neurotransmission in the culture of neuroblastoma IMR-32 cells using qPCR method.”

Human neuroblastoma IMR-32 cell line

“The human neuroblastoma cell line, IMR-32, was chosen for study because these cells express functional GABAA receptors (Anderson et al., 1993; Noble et al., 1993; Sapp and Yeh, 2000).”

Selank alone did not cause significant changes in the mRNA levels of the studied genes

“It should be noted that there were no changes in the mRNA levels of the genes studied under the effect of Selank in the IMR-32 cells.”

The combination of GABA and Selank nearly completely suppressed the gene expression changes induced by GABA alone

“The combined effect of GABA and Selank led to nearly complete suppression of changes in expression of genes in which mRNA levels changed under the effect of GABA.”

The combination of Selank and olanzapine resulted in more pronounced gene expression alterations compared to olanzapine alone

“When Selank was used in conjunction with olanzapine, the expression alterations of more genes were observed compared with olanzapine alone.”

The subunit composition of GABAA receptors in the IMR-32 cell line is not precisely defined, which may limit the interpretation of direct receptor interactions

“Currently, the subunit composition of GABAA receptors present in neuroblastoma IMR-32 cell culture is not precisely defined.”