Epitalon · research summary

AEDG Peptide (Epitalon) Stimulates Gene Expression and Protein Synthesis during Neurogenesis: Possible Epigenetic Mechanism.

Molecules (Basel, Switzerland) · 2020. Summary drawn from the study's full text. Each statement is followed by the exact sentence it comes from. This is a research summary, not medical advice.

Study type

In vitro study using human gingival mesenchymal stem cells and molecular modelling
“The aim of the study was to investigate the influence of AEDG peptide on neurogenic differentiation gene expression and protein synthesis in human gingival mesenchymal stem cells, and to suggest the basis for the epigenetic mechanism of this process.”

Subjects

Human gingival mesenchymal stem cells (hGMSCs) derived from healthy volunteers
“In the present study both types of these cells, collected from healthy volunteers (five men in the age range from 20 to 30), were first studied for their stemness characteristics.”

Sample size

Five male donors aged 20 to 30
“In the present study both types of these cells, collected from healthy volunteers (five men in the age range from 20 to 30), were first studied for their stemness characteristics.”

Reported findings

AEDG peptide increased the mRNA expression of neurogenic differentiation markers Nestin, GAP43, β Tubulin III, and Doublecortin by 1.6 to 1.8 times
“AEDG peptide increased Nestin, GAP43, β Tubulin III and Doublecortin mRNA expression by 1.6–1.8 times in hGMSCs.”
Molecular modelling indicated that AEDG peptide preferably binds to histones H1/6 and H1/3 at sites that interact with DNA
“Molecular modelling method showed, that AEDG peptide preferably binds with H1/6 and H1/3 histones in His-Pro-Ser-Tyr-Met-Ala-His-Pro-Ala-Arg-Lys and Tyr-Arg-Lys-Thr-Gln sites, which interact with DNA.”
The binding of AEDG peptide to histones H1/3 and H1/6 is suggested as a mechanism for increased transcription of neuronal differentiation genes
“AEDG peptide and histones H1/3, H1/6 binding may be one of the mechanisms which provides an increase of Nestin, GAP43, β Tubulin III, and Doublecortin neuronal differentiation gene transcription.”

Source: PubMed 32019204 · DOI 10.3390/molecules25030609 · all study summaries

Study type

In vitro study using human gingival mesenchymal stem cells and molecular modelling

“The aim of the study was to investigate the influence of AEDG peptide on neurogenic differentiation gene expression and protein synthesis in human gingival mesenchymal stem cells, and to suggest the basis for the epigenetic mechanism of this process.”

Reported findings

AEDG peptide increased the mRNA expression of neurogenic differentiation markers Nestin, GAP43, β Tubulin III, and Doublecortin by 1.6 to 1.8 times

“AEDG peptide increased Nestin, GAP43, β Tubulin III and Doublecortin mRNA expression by 1.6–1.8 times in hGMSCs.”

Molecular modelling indicated that AEDG peptide preferably binds to histones H1/6 and H1/3 at sites that interact with DNA

“Molecular modelling method showed, that AEDG peptide preferably binds with H1/6 and H1/3 histones in His-Pro-Ser-Tyr-Met-Ala-His-Pro-Ala-Arg-Lys and Tyr-Arg-Lys-Thr-Gln sites, which interact with DNA.”

The binding of AEDG peptide to histones H1/3 and H1/6 is suggested as a mechanism for increased transcription of neuronal differentiation genes

“AEDG peptide and histones H1/3, H1/6 binding may be one of the mechanisms which provides an increase of Nestin, GAP43, β Tubulin III, and Doublecortin neuronal differentiation gene transcription.”