Ghrelin alleviates intestinal ischemia-reperfusion injury by activating the GHSR-1α/Sirt1/FOXO1 pathway.

In vivo mouse model and in vitro Caco-2 cell study

“To investigate this, we established IIR by using a non-invasive arterial clip to clamp the root of the superior mesenteric artery (SMA) in mice.”

Mice and Caco-2 cells

“We mimicked the IIR process with hypoxia-reoxygenation (HR) in Caco-2 cells, which are similar to intestinal epithelial cells in structure and biochemistry.”

Ghrelin inhibited IIR/HR-induced oxidative stress and apoptosis by activating GHSR-1α

“Our results showed that ghrelin inhibited IIR/HR-induced oxidative stress and apoptosis by activating GHSR-1α.”

Ghrelin activated the GHSR-1α/Sirt1/FOXO1 signaling pathway

“Moreover, it was found that ghrelin activated the GHSR-1α/Sirt1/FOXO1 signaling pathway.”

Sirt1 was critical for ghrelin-mediated mitigation of IIR/HR injury

“We further inhibited Sirt1 and found that Sirt1 was critical for ghrelin-mediated mitigation of IIR/HR injury.”