GHRP-6 · research summary

GHSR gene knockout alleviates the liver pathological response in Echinococcus granulosus infection by reducing parasite survival.

Veterinary research · 2025. Summary drawn from the study's full text. Each statement is followed by the exact sentence it comes from. This is a research summary, not medical advice.

Study type

In vivo animal study using proteomic and metabolomic analysis in mice
“In this study, we extracted liver tissues from E. granulosus-infected mice and those treated with the GHSR antagonist [D-Lys3]-GHRP-6.”

Subjects

Female C57BL/6 mice, including wild-type and GHSR-knockout strains
“In this study, 6- to 8-week-old female C57BL/6 mice weighing 20 ± 2 g were provided by Shanghai Southern Model Biology Technology Co., Ltd.”

Sample size

18 mice total (6 per group across three groups: control, E. granulosus infected, and GHSR-knockout infected)
“The mice were subsequently divided into three groups (n = 6) on the basis of the intervention: the control group (healthy C57BL/6 mice), the E. granulosus group (C57BL/6 mice infected with E. granulosus in the liver), and the E. granulosus (GHSR−/−) group (GHSR knockout C57BL/6 mice infected with E. granulosus).”

Duration

90 days
“The experiment lasted for 90 days, with euthanasia performed at the end of the experiment.”

Reported findings

GHSR knockout resulted in a significant reduction in the number of liver infection foci compared to the infected wild-type group.
“Compared with the E. granulosus (Eg) group, the GHSR-KO group presented a significant reduction in the number of liver infection foci.”
Proteomic and metabolomic analyses identified 341 differentially expressed proteins and 101 differentially expressed metabolites in liver tissues.
“Proteomic analysis revealed 341 differentially expressed proteins, of which 185 were upregulated and 156 were downregulated.”
GHSR knockout mice exhibited lower ghrelin levels and a cytokine profile similar to controls, with reduced proinflammatory and increased anti-inflammatory cytokine secretion.
“In contrast, the GHSR-KO group presented significantly lower ghrelin levels in both the serum and liver, with reduced proinflammatory cytokine secretion and increased anti-inflammatory cytokine secretion, similar to those of the control group.”

Source: PubMed 40065480 · DOI 10.1186/s13567-025-01478-z · all study summaries

Sample size

18 mice total (6 per group across three groups: control, E. granulosus infected, and GHSR-knockout infected)

“The mice were subsequently divided into three groups (n = 6) on the basis of the intervention: the control group (healthy C57BL/6 mice), the E. granulosus group (C57BL/6 mice infected with E. granulosus in the liver), and the E. granulosus (GHSR−/−) group (GHSR knockout C57BL/6 mice infected with E. granulosus).”

Reported findings

GHSR knockout resulted in a significant reduction in the number of liver infection foci compared to the infected wild-type group.

“Compared with the E. granulosus (Eg) group, the GHSR-KO group presented a significant reduction in the number of liver infection foci.”

Proteomic and metabolomic analyses identified 341 differentially expressed proteins and 101 differentially expressed metabolites in liver tissues.

“Proteomic analysis revealed 341 differentially expressed proteins, of which 185 were upregulated and 156 were downregulated.”

GHSR knockout mice exhibited lower ghrelin levels and a cytokine profile similar to controls, with reduced proinflammatory and increased anti-inflammatory cytokine secretion.

“In contrast, the GHSR-KO group presented significantly lower ghrelin levels in both the serum and liver, with reduced proinflammatory cytokine secretion and increased anti-inflammatory cytokine secretion, similar to those of the control group.”