Effect of ACTH4-10Pro8-Gly9-Pro10 on anti-inflammatory cytokine (IL-4, IL-10, IL-13) expression in acute spinal cord injury models (male Sprague Dawley rats).

Experimental animal study using a rat model of spinal cord injury

“We carried out an experimental study in 27 male Sprague Dawley rats weighing 250-300 g, we severed the spinal cords in the area of injury, then we fixed the specimen with formalin.”

Male Sprague Dawley rats

“In this study we used 12-week-old male Sprague Dawley rats weighing 250-300 g to reproduce homogenous sample for SCI models and eliminate confounding factor such as sex hormone.”

Termination at 3 and 6 hours post-injury

“Each group was divided again into two groups to be respectively terminated at 3 hours and 6 hours after compression.”

In severe SCI, ACTH 4-10 resulted in higher levels of IL-10 and IL-13 at 3 hours, and IL-4 and IL-10 at 6 hours compared to placebo

“Rats with severe compression in ACTH 4-10 group showed greater levels of IL-10, IL-13 at 3 hours and IL-4, IL-10 at 6 hours compared with the placebo group.”