GHRP-6 · research summary

Effect of intracerebroventricular (ICV) injection of antimicrobial peptide expressed in the body-2 (LEAP-2) and its interaction with cannabinoid and ghrelin systems on food intake in broiler chickens.

Poultry science · 2026. Summary drawn from the study's full text. Each statement is followed by the exact sentence it comes from. This is a research summary, not medical advice.

Study type

Experimental study investigating the effect of intracerebroventricular (ICV) injection of LEAP-2 and its interaction with cannabinoid and ghrelin systems on food intake.
“This study aimed to determine effect of ICV injection of LEAP2and its interaction with cannabinoid and ghrelin systems on food intake in broiler chickens.”

Subjects

One-day-old Arian strain broiler chicks.
“A total of 176 broiler chicks, one day old and of the Arian strain, were obtained from Mahan Company, a hatchery located in Tehran, Iran.”

Sample size

176 broiler chicks divided into 4 experiments with 4 groups of 11 chicks each.
“In the present study 4 experiments were conducted, each containing 4 experimental groups with 11 chicks per group (total n = 176).”

Duration

Food intake was measured at intervals of 30, 60, and 120 minutes post-administration.
“The cumulative meal consumption was subsequently measured at intervals of 30, 60, and 120 min post-administration.”

Reported findings

Co-injection of the ghrelin antagonist (D-Lys-3)-GHRP-6 with LEAP-2 significantly decreased LEAP-2-induced hypophagia.
“Co-injection of the (D-Lys-3)-GHRP-6 + LEAP2 significantly decreased LEAP2-induced hypophagia (P<0.05).”
Co-injection of CB1 antagonist SR141716A or CB2 antagonist AM630 with LEAP-2 significantly amplified LEAP-2-induced hypophagia.
“Co-injection of the SR141716A + LEAP2 significantly amplified LEAP2-induced hypophagia compared to control group (P<0.05).”

Limitations stated by the authors

The study used a specific broiler strain (Arian), and the authors note that differences in central food intake regulation may exist between this strain and other commercial strains like ROSS-308.
“In this study, Arian strain broiler chicks were utilized, and there may be a difference in the regulation of central food intake between Arian strain and ROSS-308.”

Source: PubMed 41406822 · DOI 10.1016/j.psj.2025.106199 · all study summaries

Study type

Experimental study investigating the effect of intracerebroventricular (ICV) injection of LEAP-2 and its interaction with cannabinoid and ghrelin systems on food intake.

“This study aimed to determine effect of ICV injection of LEAP2and its interaction with cannabinoid and ghrelin systems on food intake in broiler chickens.”

Reported findings

Co-injection of the ghrelin antagonist (D-Lys-3)-GHRP-6 with LEAP-2 significantly decreased LEAP-2-induced hypophagia.

“Co-injection of the (D-Lys-3)-GHRP-6 + LEAP2 significantly decreased LEAP2-induced hypophagia (P<0.05).”

Co-injection of CB1 antagonist SR141716A or CB2 antagonist AM630 with LEAP-2 significantly amplified LEAP-2-induced hypophagia.

“Co-injection of the SR141716A + LEAP2 significantly amplified LEAP2-induced hypophagia compared to control group (P<0.05).”

Limitations stated by the authors

The study used a specific broiler strain (Arian), and the authors note that differences in central food intake regulation may exist between this strain and other commercial strains like ROSS-308.

“In this study, Arian strain broiler chicks were utilized, and there may be a difference in the regulation of central food intake between Arian strain and ROSS-308.”