Efficient Expression of Small Molecule Bioactive Peptides in Bacillus licheniformis.

In vitro fermentation optimization and in vivo rat model assessment

“Biological activity was assessed in a rat model of ethanol-induced acute gastric ulcer.”

Bacillus licheniformis strain 2709 and rats

“Stable expression of γ-Glutamyltranspeptidase (GGT)-BPC157 and mScarlet-BPC157 in Bacillus licheniformis strain 2709 by chromosomal integration.”

Reporter-based expression signals increased approximately threefold under optimal fermentation conditions

“Under optimal conditions (2% inoculum, 40 g/L soybean peptone, and 80 g/L glucose), reporter-based expression signals increased approximately threefold compared to the basal medium.”

Fusion proteins showed gastroprotective and therapeutic activity with ulcer inhibition rates ranging from 75.3% to 84.6%

“Both proteins showed gastroprotective and therapeutic activity, with ulcer inhibition rates ranging from 75.3% to 82.9% protective regime and 80.4% to 84.6% therapeutic regime.”

Treatment reduced mucosal inflammation, stimulated glandular repair, and downregulated proinflammatory cytokines

“Histological analysis showed that the treatment reduced mucosal inflammation and stimulated glandular repair.”