NAD+ · research summary

Integrated assessment of fatty acid metabolism and cellular energy status using HILIC-MS/MS.

bioRxiv : the preprint server for biology · 2026. Summary drawn from the study's full text. Each statement is followed by the exact sentence it comes from. This is a research summary, not medical advice.

Study type

Method development and application study
“Here, we describe a convenient method based on hydrophilic interaction liquid chromatography (HILIC-MS/MS) for the concurrent detection of both short-chain and long-chain acyl-CoA and their corresponding acyl-carnitine species.”

Subjects

Multiple cell lines
“Using this strategy, we tracked the metabolism of isotope-labeled fatty acids in multiple cell lines.”

Reported findings

Differences in fatty acid oxidation propensities were observed across cell lines
“Using this strategy, we tracked the metabolism of isotope-labeled fatty acids in multiple cell lines, which revealed differences in their propensities for fatty acid oxidation.”
Isotope incorporation into acyl-CoA did not always mirror that of acyl-carnitine
“Using this strategy, we tracked the metabolism of isotope-labeled fatty acids in multiple cell lines, which revealed differences in their propensities for fatty acid oxidation and the extent to which isotope incorporation into acyl-CoA mirrored that of acyl-carnitine.”
The workflow enabled analysis of NADH and ATP to gauge cellular bioenergetics
“We also applied the HILIC-MS/MS workflow to the analysis of NADH and ATP, making it a useful technique for gauging cellular bioenergetics as reflected by the acetyl-CoA/CoA, NADH/NAD + , and ATP/ADP ratios.”

Source: PubMed 42845436 · DOI 10.64898/2026.08.12.744242 · all study summaries