SIRT6 promotes a mesenchymal, invasive phenotype in melanoma.

In vitro experimental study using melanoma cell lines

“The experimental framework primarily relies on in vitro melanoma cell models.”

SK-MEL-3 and A375 human melanoma cell lines

“SK-MEL-3 human melanoma cell lines were purchased from ATCC and maintained in McCoy’s 5 A medium (Corning #10-050-CV) supplemented with 15% FBS (Sigma-Aldrich #F2442).”

Not specified in terms of number of cells or replicates beyond technical/biological replicates

“Data are shown as mean ± SEM with at least 2 biological replicates, and at least 3 technical replicates unless otherwise specified.”

Variable durations for specific assays ranging from 24 hours to approximately 10 days

“Cells were maintained under standard tissue culture conditions, changing media every 3 days for approximately 10 days, until the cells reached optimal density.”

SIRT6 overexpression increased cell growth, viability, and colony formation

“Our data demonstrated that SIRT6 OE significantly increased cell growth and viability in SK-MEL-3 cells as compared to the pcDNA empty-vector control cells (Figure 1B).”

SIRT6 overexpression upregulated mesenchymal markers and EMT transcriptional regulators

“We showed that SIRT6 overexpression significantly increased expression of the mesenchymal markers N-cadherin, vimentin, and β-catenin as compared to the pcDNA empty vector control cells (Figure 2A).”

SIRT6 knockdown inhibited cell migration and invasion

“The wound healing scratch assay showed marked inhibition of cell migration following SIRT6 KD in the melanoma cells (Figure 4A).”

Reliance on in vitro models and specific cell lines with distinct mutations

“The experimental framework primarily relies on in vitro melanoma cell models.”